ʻO ka mea mua, ʻo ka matrine kahi pesticide i loaʻa i nā mea kanu me nā ʻano kikoʻī a kūlohelohe hoʻi. Hoʻopili wale ia i nā meaola kikoʻī a hiki ke hoʻopau koke ʻia i ke ʻano. ʻO ka huahana hope loa ʻo carbon dioxide a me ka wai. ʻO ka lua, ʻo ka matrine kahi endogenous mea kanu Nine Inch i hana kūʻē i nā meaola weliweli. ʻAʻole kū hoʻokahi ka hoʻohui, akā hoʻohui o nā hui he nui me nā hanana kemika like a me nā hui he nui me nā ʻano kemika ʻokoʻa, kahi e hoʻopili ai kekahi i kekahi a pāʻani pū kekahi i ka hana. ʻO ke kolu, hiki ke hoʻohana ʻia ka matrine no ka manawa lōʻihi ma muli o ka hana hui ʻana o nā ʻano mea like ʻole o ka kemika, e paʻakikī ana i ke kūʻē ʻana i nā mea ʻino. ʻO ka hā, ʻaʻole e hoʻopōʻino ʻia nā mea make e like me ka pololei, akā ʻo ka kaohi ʻana i ka heluna o nā mea pests ʻaʻole ia e hoʻopili nui i ka hana a me ka hana hou ʻana i ka heluna mea kanu. ʻAno like kēia ʻano hana me ke kumumanaʻo o ka kaohi hama i ka pale ākea a me ka ʻōnaehana hoʻomalu i hoʻomohala ʻia ma hope o nā makahiki he umi o ka noiʻi ma hope o ka lilo ʻana o nā hopena ʻaoʻao o ka pale pesticide kemika i mea nui. Hiki i nā helu ʻehā ma luna ke hōʻike i ka ʻokoʻa o ka matrine mai ka nui-ʻona nui a me nā koena pesticides kemikala kiʻekiʻe, a he ʻōmaʻomaʻo loa a ʻoluʻolu kūlohelohe.